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Dec 21, 2024
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BIOL 0565 - Biotechnology
Credits: 4
Semester: 2nd Semester.
Lecture: 2
Lab: 6
This course is designed to introduce advanced undergraduates and graduate students to basic recombinant DNA techniques including growth and manipulation of phages and their bacterial hosts; isolation, quantitation, and electrophoretic analysis of DNA; restriction and ligation of DNA cloning in lambda, M13 and plasmid vectors; and site-specific mutagenesis. The focus of the course is hands-on experimentation; however, time will be devoted to discussion of the application of these and other techniques to a variety of research problems. By the end of the course, the student should have a working knowledge of basic recombinant technology, should have an introductory knowledge of more specialized techniques and should be familiar with the terminology and resource literature of genetic engineering.
Prerequisite(s): BIOL 0230 /BIOL 0231 , BIOL 0250 /BIOL 0251 , BIOL 0301 /BIOL 0303 .
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